LOURIDO LAB

Whitehead Institute for Biomedical Research

Welcome

Intracellular parasites expose the extraordinary diversity and range of eukaryotic cell biology. Our lab seeks to understand the intimate host–parasite interactions that support infection at multiple scales, from their molecular origins to their systemic consequences. Our primary model for this work has been Toxoplasma gondii, a member of the Apicomplexa, a group of ubiquitous protist parasites that has co-evolved with animals for hundreds of millions of years. Toxoplasma and related apicomplexans are responsible for many human and veterinary diseases, including toxoplasmosis, malaria, and cryptosporidiosis. Using genetic screens, quantitative cell biology, biochemistry, proteomics, and emerging systems-level approaches, we investigate the molecular machinery that allows these parasites to invade cells, sense and respond to their environment, regulate gene expression and metabolism, and establish persistent infections. Increasingly, our work extends to the host–parasite interactions that mediate these processes and their broader implications in disease. By uncovering both the conserved and uniquely adapted biology of these organisms, we have revealed new principles of eukaryotic biology and found new opportunities for therapeutic intervention.

The Lourido Lab, 2026

Lab News

  • Toxoplasma goes PRO

    August 11, 2026. Chris and Chinmay's new paper on how Toxoplasma adapts its metabolism to oxidative stress is now live at Cell. The study details how a novel RNA-binding protein we called TgPRO, stabilizes specific transcripts to mediate the adaptation. This was a great collaboration with colleagues in the Harding, Hunter, and Soldati-Favre labs.

  • Simon wins best talk award

    June 17, 2026. Simon Butterworth received an award for one of the best oral presentations at the 18th International Congress on Toxoplasmosis, held in Rio de Janeiro, Brazil. His presentation showcased recent advances in crosslinking mass spectrometry.

  • Zach receives Burroughs Wellcome Fund Postdoctoral Enrichment Program award

    June 10, 2026. Zachary Sanchez received the Burroughs Wellcome Fund and The Charles H. Revson Foundation 2026 Postdoctoral Enrichment Program (PDEP) award, providing career-development support to postdoctoral researchers. Congratulations Zach!

  • New lab member!

    June 2026. Tori Sodeinde (grad student in Biology) joins our lab to work with our growing interactomics team.

  • Welcome new lab members and summer students

    June 2025. We are excited to welcome our newest lab members: Chris Pukszta (grad student in Biology) and Zachary Sanchez (postdoc)! Three undergrads are also visiting for the summer: Jariatu Kargbo (from UMBC), Sam Choi (from UCLA), and Tom Duraisingh (from UCSD).

  • Sebastian receives 2025 Science and Engineering Outstanding Alumni Award for Professional Excellence from Tulane

    April 2025. Congratulations, Sebastian!

  • Chris wins Regeneron Prize

    August 1, 2024. Congratulations to Chris for winning the Regeneron Prize for Creative Innovation! Read more about the competition and his winning proposal on leveraging Toxoplasma for muscle-directed gene therapy here.

  • Welcome new lab members!

    June 24, 2024. The lab is excited to welcome 3 new members for the summer and beyond: Kenneth Wei, a graduate student in HST; Ana Karla Cepeda Diaz, a postdoctoral fellow; and Jariatu Kargbo, an undergraduate student from UMBC participating in MSRP.

  • Congratulations to our 2024 grads Mudita, Sundeep, and Zoe!

    May 31, 2024. Wishing our fantastic undergrads good luck as they embark on their next adventures. We can’t wait to hear about all the amazing science you’ll do as researchers and doctors!

  • ToxoXVII Berlin

    May 30, 2024. Sebastian, Eden, Chris, Dylan, and Michelle enjoyed a great Toxoplasma Congress in Berlin. Congratulations to all for their presentations and especially Chris Giuliano for winning a best talk award!

  • Haley receives Kirschstein-NRSA postdoctoral fellowship

    April 1, 2024. Congratulations to Haley Licon for earning the Ruth L. Kirschstein Postdoctoral Individual National Research Service Award!

  • HiT the Toxoplasma genome

    April 28, 2022. Tyler’s paper on the high-throughput tagging (HiT) of Toxoplasma genes is now published in Nature Microbiology. The paper describes a new method of CRISPR-based screening that manipulates genes through tagging instead of disrupting. Using this approach, we discovered a new regulator of the parasite lytic cycle.

  • Dr. Tyler A. Smith

    April 14, 2022. Tyler successfully defends his thesis on the High-throughput functionalization of the Toxoplasma kinome. Congratulations Dr. Smith!

  • Dylan receives NSF fellowship

    April 14, 2022. Congratulations to Dylan McCormick for receiving one of the National Science Foundation Graduate Research Fellowship Program awards!

  • Positive feedback

    April 7, 2022. Haley’s manuscript describing the discovery of BFD2 and the positive-feedback loop that regulates chronic differentiation of Toxoplasma is out on bioRxiv.

  • Alex on the Biogenesis podcast

    December 2, 2021. Alex was featured in the Biology Department’s Biogenesis podcast, sharing his journey to our lab. Very proud to have you as a lab member, Alex!

  • TPP protocol published

    November 9, 2021. Alice’s Thermal Proteome Profiling protocol is now available on Bio-Protocol.

  • CRISPRi in Toxoplasma

    October 14, 2021. Benedikt’s final installment on the development of wonderful molecular tools for Toxoplasma describes the adaptation of CRISPRi technology to the parasite. His paper in mSphere describes promising evidence for the downregulation of parasite gene expression through targeting of a dead Cas9 to the promoter.

  • BWF PATH Award

    May 20, 2021. The Burroughs Wellcome Fund announces Sebastian as one of its new Investigators in the Pathogenesis of Infectious Disease, supporting the lab’s work on host-parasite interactions for five years.

  • Michelle receives honorable mention

    March 23, 2021. Congratulations to Michelle for receiving an honorable mention for her proposal to the National Science Foundation Graduate Research Fellowship Program.

  • The tenure track

    February 19, 2021. Congratulations to Sebastian for his promotion to Associate Professor in the Biology Department at MIT.

  • Where genes start!

    January 20, 2021. Benedikt’s paper mapping transcriptional start sites across the Toxoplasma genome is out in Frontiers in Cellular & Infection Microbiology. An incredible dataset for the community and some amazing observations about Toxoplasma transcription.

  • Tyler awarded best talk at MPM

    October 22, 2020. Tyler received one of the best talk awards at the XXXI Molecular Parasitology Meeting, in Woods Hole, MA.

  • The Art of killing parasites

    September 23, 2020. Clare, Saima, and Bory’s paper on the mechanisms of Artemisinin resistance and susceptibility in Toxoplasma is out in Nature Communications. The study finds that altering the levels of heme in parasites can increase or decrease their susceptibility to the important antimalarial compound.

  • Identifying drug targets in Toxoplasma

    July 5, 2020. Alice’s paper is out! Using thermal proteome profiling, Alice identified the targets of a previously identified compound that modulates parasite calcium signalling. Read more at WI news.

  • Another Ben defends

    May 6, 2020. Ben Waldman successfully defends his thesis (over Zoom). With almost 100 colleagues, friends, and family members logged in for the event, Ben walks us through his masterful work on the regulation of chronic toxoplasmosis (and a few digital challenges). Congratulations Dr. Ben!

  • Ben defends

    March 6, 2020. Benedikt Markus travels back to Germany to defend his thesis on Toxoplasma genome engineering and genomics before the faculty of the University of Freiburg, friends, and family. Congratulations Dr. Markus!

  • How parasites persist

    January 2020. Ben’s paper on the master regulator of Toxoplasma chronic differentiation is now available online!

  • Gabriella receives award at ABRCMS

    November 17, 2019. Showcasing the work she completed during her semester as an undergraduate researcher in our lab, Gabriella received a Presentation Award during the Annual Biomedical Research Conference for Minority Students. Next stop graduate school!

  • Lab members complete half marathon

    November 3, 2019. Liz, Ben, Tyler and Sebastian all survived and completed the Cambridge Half Marathon, collecting the first medals of the Lourido Lab Running Club.

  • Ben & Tyler receive MPM awards

    October 23, 2019. For their presentations at the Molecular Parasitology Meeting in Woods Hole, Ben and Tyler received awards for Best Full Length Talk and Best Poster, respectively. As part of the award, Ben will present his work at the annual meeting of the American Society for Tropical Medicine and Hygiene.

  • Optimizing Cas9 for Toxoplasma

    June 26, 2019. Benedikt’s paper describing improvements to the expression of Cas9 in Toxoplasma is out! New expression plasmid available from Addgene.

  • Sebastian recognized by the University of Quindío

    June 21, 2019. Sebastian receives a special recognition from the University of Quindío for his contributions to the field at the international level. The recognition was received as part of the 15th International Congress on Toxoplasmosis.

  • Alice and Alex awarded Graduate Fellowships

    May 15, 2019. Alice Herneisen and Alex Chan receive prestigious Graduate Research Fellowships from the National Science Foundation.

  • Diego to start his lab at UGA

    May 3, 2019. Diego has been recruited to be an Assistant Professor at the College of Pharmacy and the Center for Tropical and Emerging Global Diseases at the University of Georgia, Athens. We look forward to many new discoveries from his new lab!

  • Clare receives Sir Henry Dale

    December 7, 2018. Clare receives the prestigious Sir Henry Dale Fellowship from the Wellcome Trust to support her new lab at the Wellcome Trust Centre for Molecular Parasitology in Glasgow, UK.

  • New Members

    May–July 2018. A much overdue welcome to several new lab members: Elizabeth Costa joins us as a postdoctoral fellow after completing her PhD with Jonathan Weissman at UCSF, Alice Herneisen and Alex Chan both join us as doctoral students in the MIT biology program, Boryana Petrova joins us as a postdoctoral fellow after completing her work with Terry Orr-Weaver, Dominic Schwarz joins us for his masters thesis from the University of Heidelberg, and Marienela Heredia is an Amgen Scholar joining us for the summer. Looking forward to working with all of them!

  • We found it!

    May 11, 2018. Diego’s paper on the cryptic stator of the apicomplexan ATP synthase is out on bioRxiv.

  • Diego receives K99

    April 17, 2018. Diego Huet receives a prestigious Pathway to Independence K99 Award from NIAID, to fund his work on Toxoplasma mitochondria and the unusual features of their ATP synthase.

  • Tyler receives a GRFP award

    April 3, 2018. Tyler Smith receives a Graduate Research Fellowship from the National Science Foundation for his proposal to functionalize the Toxoplasma proteome.

  • Clare wins Koch Institute image award

    March 8, 2018. Clare unveils her beautiful image of Toxoplasma at this year’s Koch Institute image awards. The 6-foot installation will be on display in the Institute’s public gallery for the next year. You can see Clare’s image and the other winners here.

  • Tammy joins the Lourido Lab

    February 16, 2018. Tammy Lan joins us from the University of Heidelberg to investigate host-parasite interactions through CRISPR-based screens in Toxoplasma. She will be completing this research over the next six months as part of her masters thesis.

  • Our CRISPR screening protocol is out!

    January 2018. Our lab’s protocol, “CRISPR-Cas9-based genome-wide screening of Toxoplasma gondii,” is now available online at Nature Protocols.

  • Host Hacking course published on OCW

    January 24, 2017. Clare and Diego’s course is now available on MIT OpenCourseWare: 7.341 Host Hacking: Parasitic Manipulations from a Micro- to a Macroscopic Scale.

  • Sebastian appointed Whitehead Member

    January 18, 2017. Starting July 1st, Sebastian will be a member of the Whitehead Institute and assistant professor of biology at MIT. Full news article.

  • Diego receives MPM Award

    October 10, 2016. Diego received an award for best talk at the 2016 Molecular Parasitology Meeting in Woods Hole, MA. As part of the award, he will be invited to present his work at the ASTMH meeting in Atlanta.

Research

Molecular Interplay at the Host–Parasite Interface

Obligate intracellular parasites like Toxoplasma depend on their hosts for survival. These parasites must recognize and invade host cells, acquire essential nutrients, and reshape their intracellular environment—all without compromising host survival prior to parasite replication and transmission. Remarkably, Toxoplasma can accomplish this across an exceptionally broad range of host species and cell types. We investigate the cellular and molecular events that enable these interactions, from the mechanisms of host cell recognition and invasion to nutrient acquisition, effector secretion, and remodeling of host organelles and pathways. By dissecting these interactions from both sides of the host–parasite interface, we seek to uncover the fundamental molecular logic that makes intracellular parasitism possible.

Infection Across Scales

The molecular mechanisms that enable infection ultimately determine how parasites disseminate through tissues, evade or withstand immunity, and persist within an animal host. We investigate how these processes operate across scales, connecting genes and cellular phenotypes to the trajectory of infection. Using genetic screens and experimental models of infection, we identify parasite factors that determine virulence, dissemination, and tissue-specific fates, while examining the host responses that promote clearance, tolerance, or persistence. By linking molecular mechanisms to organismal outcomes, we aim to understand how parasites establish long-term relationships with their hosts and how those relationships shape both pathogenesis and immunity.

Proteome Organization and Dynamics

Cellular behavior emerges not only from which genes are expressed, but from how proteins are modified, assembled, and reorganized in space and time. We use quantitative proteomics and mass spectrometry to uncover this dynamic layer of cell biology, combining approaches such as affinity purification, proximity labeling, cross-linking mass spectrometry (XL-MS), and analysis of post-translational modifications. These methods allow us to map protein interactions and molecular architecture at scales ranging from individual complexes to entire cells and to determine how they change as parasites transition between cellular states. In particular, advances in XL-MS allow us to capture protein–protein interactions, at scale, which provide critical empirical constraints for AI-based structural modeling. We also develop approaches that extend these measurements to genetically inaccessible parasites and their free-living relatives, using comparative proteome organization to explore how the molecular machinery of parasitism evolved.

Cellular Plasticity and Adaptation

Parasites encounter dramatically different environments over the course of infection, from brief periods outside cells to replication within diverse host tissues and long-term persistence in chronic stages. Survival therefore requires an extraordinary capacity to sense changing conditions and adopt new cellular states. We investigate the signaling pathways and gene-regulatory mechanisms that give Toxoplasma and related parasites this plasticity, asking how environmental information is sensed, transmitted, and ultimately translated into changes in gene expression, metabolism, growth, and developmental fate. These studies reveal both conserved principles of eukaryotic regulation and unusual molecular solutions that have evolved to support the complex life cycles of parasites.

Lab Members

Staff

Undergraduate Students

Graduate Students

Postdoctoral Fellows

Support Team

  • Kafka Kafka Dog
  • Cat Cat Cat
  • Chip & Dale Chip & Dale Cats
  • Olive Olive Cat
  • Willow Willow Cat
  • Miso Miso Cat
  • Callie Callie Dog

Publications

2026 — 3 publications

  • Convergent evolution of metabolic regulation governs redox adaptation in Toxoplasma.

    Giuliano CJ, Kalluraya CA, Kloehn J, Sloan MA, Bunkofske ME, Hunter CA, Soldati-Favre D, Harding CR, Lourido S.

    Cell. 2026 Aug 11:S0092-8674(26)00827-5.

    PMID: 42580337

  • Brieflow: an integrated computational pipeline for high-throughput analysis of optical pooled screening data.

    Di Bernardo M, Kern RS, Cepeda Diaz AK, Mallar A, Choi SJ, Nutter-Upham A, Lourido S, Blainey PC, Cheeseman I.

    Nat Commun. 2026 May 30;17(1):6997.

    PMID: 42218140

  • Two distinct SWI/SNF complexes direct chromatin-linked transcriptional programs in Toxoplasma.

    Schwarz D, Tapia B, Lourido S.

    Nat Commun. 2026 May 15;17(1):6456.

    PMID: 42140969

2025 — 8 publications

  • Brain-immune interactions generate pathogen-specific sickness states.

    Sullivan ZA, Wong BJ, Kapoor V, Vincze PK, Kaplan HS, Giraudet P, Watson BR, Misherghi A, Lourido S, Moffitt JR, Dulac C.

    bioRxiv [Preprint]. 2025 Dec 8:2025.12.06.692770.

    PMID: 41427305

  • Clustering of host N-glycans licenses Toxoplasma rhoptry discharge.

    Valleau D, Goyal M, Roberts JM, Male F, Ward G, Lourido S.

    bioRxiv [Preprint]. 2025 Oct 21:2025.10.16.682961.

    PMID: 41279910

  • Alternative start codon selection shapes mitochondrial function and rare human diseases.

    Ly J, Di Bernardo M, Tao YF, Khalizeva E, Giuliano CJ, Lourido S, Fleming MD, Cheeseman IM.

    Mol Cell. 2025 Nov 20;85(22):4198-4214.e11.

    PMID: 41205602

  • Perforation of the host cell plasma membrane during Toxoplasma invasion requires rhoptry exocytosis.

    Male F, Kegawa Y, Blank PS, Jiménez-Munguía I, Sidik SM, Valleau D, Lourido S, Lebrun M, Zimmerberg J, Ward GE.

    EMBO Rep. 2025 Oct;26(20):5027-5047.

    PMID: 40973827

  • 5′ untranslated regions tune Toxoplasma translation.

    Peters ML, Shukla A, Gotthold ZA, McCormick DM, Xiang K, Bartel DP, Lourido S.

    bioRxiv [Preprint]. 2025 Jul 14:2025.07.14.664749.

    PMID: 40791393

  • Metabolic adaptability and nutrient scavenging in Toxoplasma gondii: insights from ingestion pathway-deficient mutants.

    Rimple PA, Olafsson EB, Markus BM, Wang F, Augusto L, Lourido S, Carruthers VB.

    mSphere. 2025 Apr 29;10(4):e0101124.

    PMID: 40172222

  • Immune targeting and host-protective effects of the latent stage of Toxoplasma gondii.

    Eberhard JN, Shallberg LA, Winn A, Chandrasekaran S, Giuliano CJ, Merritt EF, Willis E, Konradt C, Christian DA, Aldridge DL, Bunkofske ME, Jacquet M, Dzierszinski F, Katifori E, Lourido S, Koshy AA, Hunter CA.

    Nat Microbiol. 2025 Apr;10(4):992-1005.

    PMID: 40148566

  • Finding the brakes on the Toxoplasma life cycle.

    Herneisen AL, Lourido S.

    Trends Parasitol. 2025 Apr;41(4):264-266.

    PMID: 40055102

2024 — 5 publications

  • TgATG9 is required for autophagosome biogenesis and maintenance of chronic infection in Toxoplasma gondii.

    Thaprawat P, Zhang Z, Rentchler EC, Wang F, Chalasani S, Giuliano CJ, Lourido S, Di Cristina M, Klionsky DJ, Carruthers VB.

    Autophagy Rep. 2024;3(1):2418256.

    PMID: 39600488

  • SPARK regulates AGC kinases central to the Toxoplasma gondii asexual cycle.

    Herneisen AL, Peters ML, Smith TA, Shortt E, Lourido S.

    eLife. 2024 Aug 13;13:RP93877.

    PMID: 39136687

  • CRISPR-based functional profiling of the Toxoplasma gondii genome during acute murine infection.

    Giuliano CJ, Wei KJ, Harling FM, Waldman BS, Farringer MA, Boydston EA, Lan TCT, Thomas RW, Herneisen AL, Sanderlin AG, Coppens I, Dvorin JD, Lourido S.

    Nat Microbiol. 2024 Sep;9(9):2323-2343.

    PMID: 38977907

  • Translation initiation factor eIF1.2 promotes Toxoplasma stage conversion by regulating levels of key differentiation factors.

    Wang F, Holmes MJ, Hong HJ, Thaprawat P, Kannan G, Huynh MH, Schultz TL, Licon MH, Lourido S, Dong W, Brito Querido J, Sullivan WJ, O'Leary SE, Carruthers VB.

    Nat Commun. 2024 May 23;15(1):4385.

    PMID: 38782906

  • Discovery of antibiotics that selectively kill metabolically dormant bacteria.

    Zheng EJ, Valeri JA, Andrews IW, Krishnan A, Bandyopadhyay P, Anahtar MN, Herneisen A, Schulte F, Linnehan B, Wong F, Stokes JM, Renner LD, Lourido S, Collins JJ.

    Cell Chem Biol. 2024 Apr 18;31(4):712-728.e9.

    PMID: 38029756

2023 — 5 publications

  • Analysis of CDPK1 targets identifies a trafficking adaptor complex that regulates microneme exocytosis in Toxoplasma.

    Chan AW, Broncel M, Yifrach E, Haseley NR, Chakladar S, Andree E, Herneisen AL, Shortt E, Treeck M, Lourido S.

    eLife. 2023 Nov 7;12:RP85654.

    PMID: 37933960

  • A conserved complex of microneme proteins mediates rhoptry discharge in Toxoplasma.

    Valleau D, Sidik SM, Godoy LC, Acevedo-Sánchez Y, Pasaje CFA, Huynh MH, Carruthers VB, Niles JC, Lourido S.

    EMBO J. 2023 Dec 1;42(23):e113155.

    PMID: 37886905

  • The multifaceted roles of Myb domain-containing proteins in apicomplexan parasites.

    Schwarz D, Lourido S.

    Curr Opin Microbiol. 2023 Dec;76:102395.

    PMID: 37866202

  • CDPK2A and CDPK1 form a signaling module upstream of Toxoplasma motility.

    Shortt E, Hackett CG, Stadler RV, Kent RS, Herneisen AL, Ward GE, Lourido S.

    mBio. 2023 Oct 31;14(5):e0135823.

    PMID: 37610220

  • A positive feedback loop controls Toxoplasma chronic differentiation.

    Licon MH, Giuliano CJ, Chan AW, Chakladar S, Eberhard JN, Shallberg LA, Chandrasekaran S, Waldman BS, Koshy AA, Hunter CA, Lourido S.

    Nat Microbiol. 2023 May;8(5):889-904.

    PMID: 37081202

2022 — 3 publications

  • Temporal and thermal profiling of the Toxoplasma proteome implicates parasite Protein Phosphatase 1 in the regulation of Ca2+-responsive pathways.

    Herneisen AL, Li ZH, Chan AW, Moreno SNJ, Lourido S.

    Elife. 2022 Aug 17;11:e80336.

    PMID: 35976251

  • A haem-sequestering plant peptide promotes iron uptake in symbiotic bacteria.

    Sankari S, Babu VMP, Bian K, Alhhazmi A, Andorfer MC, Avalos DM, Smith TA, Yoon K, Drennan CL, Yaffe MB, Lourido S, Walker GC.

    Nat Microbiol. 2022 Sep;7(9):1453-1465.

    PMID: 35953657

  • Screening the Toxoplasma kinome with high-throughput tagging identifies a regulator of invasion and egress.

    Smith TA, Lopez-Perez GS, Herneisen AL, Shortt E, Lourido S.

    Nat Microbiol. 2022 Jun;7(6):868-881.

    PMID: 35484233

2021 — 4 publications

  • Toxoplasma gondii exploits the host ESCRT machinery for parasite uptake of host cytosolic proteins.

    Rivera-Cuevas Y, Mayoral J, Di Cristina M, Lawrence AE, Olafsson EB, Patel RK, Thornhill D, Waldman BS, Ono A, Sexton JZ, Lourido S, Weiss LM, Carruthers VB.

    PLoS Pathog. 2021 Dec 13;17(12):e1010138.

    PMID: 34898650

  • Thermal Proteome Profiling to Identify Protein-ligand Interactions in the Apicomplexan Parasite Toxoplasma gondii.

    Herneisen AL, Lourido S.

    Bio Protoc. 2021 Nov 5;11(21):e4207.

    PMID: 34859122

  • CRISPR-Mediated Transcriptional Repression in Toxoplasma gondii.

    Markus BM, Boydston EA, Lourido S.

    mSphere. 2021 Oct 27;6(5):e0047421.

    PMID: 34643425

  • High-Resolution Mapping of Transcription Initiation in the Asexual Stages of Toxoplasma gondii.

    Markus BM, Waldman BS, Lorenzi HA, Lourido S.

    Front Cell Infect Microbiol. 2021 Jan 20;10:617998.

    PMID: 33553008

2020 — 8 publications

  • Genome-wide screens identify Toxoplasma gondii determinants of parasite fitness in IFNγ-activated murine macrophages.

    Wang Y, Sangaré LO, Paredes-Santos TC, Hassan MA, Krishnamurthy S, Furuta AM, Markus BM, Lourido S, Saeij JPJ.

    Nat Commun. 2020 Oct 16;11(1):5258.

    PMID: 33067458

  • Genetic screens reveal a central role for heme metabolism in artemisinin susceptibility.

    Harding CR, Sidik SM, Petrova B, Gnädig NF, Okombo J, Herneisen AL, Ward KE, Markus BM, Boydston EA, Fidock DA, Lourido S.

    Nat Commun. 2020 Sep 23;11(1):4813.

    PMID: 32968076

  • Identifying the Target of an Antiparasitic Compound in Toxoplasma Using Thermal Proteome Profiling.

    Herneisen AL, Sidik SM, Markus BM, Drewry DH, Zuercher WJ, Lourido S.

    ACS Chem Biol. 2020 Jul 17;15(7):1801-1807.

    PMID: 32597628

  • A PEROXO-Tag Enables Rapid Isolation of Peroxisomes from Human Cells.

    Ray GJ, Boydston EA, Shortt E, Wyant GA, Lourido S, Chen WW, Sabatini DM.

    iScience. 2020 May 22;23(5):101109.

    PMID: 32417403

  • Functional and Computational Genomics Reveal Unprecedented Flexibility in Stage-Specific Toxoplasma Metabolism.

    Krishnan A, Kloehn J, Lunghi M, Chiappino-Pepe A, Waldman BS, Nicolas D, Varesio E, Hehl A, Lourido S, Hatzimanikatis V, Soldati-Favre D.

    Cell Host Microbe. 2020 Feb 12;27(2):290-306.e11.

    PMID: 31991093

  • Identification of a master regulator of differentiation in Toxoplasma.

    Waldman BS, Schwarz D, Wadsworth MS 2nd, Saeij JP, Shalek AK, Lourido S.

    Cell. 2020 Jan 23;180(2):359-372.e16.

    PMID: 31955846

  • Plate-Based Quantification of Stimulated Toxoplasma Egress.

    Shortt E, Lourido S.

    Methods Mol Biol. 2020;2071:171-186.

    PMID: 31758453

  • High-Throughput Measurement of Microneme Secretion in Toxoplasma gondii.

    Brown KM, Sibley LD, Lourido S.

    Methods Mol Biol. 2020;2071:157-169.

    PMID: 31758452

2019 — 5 publications

  • In Vivo CRISPR Screen Identifies TgWIP as a Toxoplasma Modulator of Dendritic Cell Migration.

    Sangaré LO, Ólafsson EB, Wang Y, Yang N, Julien L, Camejo A, Pesavento P, Sidik SM, Lourido S, Barragan A, Saeij JPJ.

    Cell Host Microbe. 2019 Oct 9;26(4):478-492.e8.

    PMID: 31600500

  • The GRA17 Parasitophorous Vacuole Membrane Permeability Pore Contributes to Bradyzoite Viability.

    Paredes-Santos T, Wang Y, Waldman B, Lourido S, Saeij JP.

    Front Cell Infect Microbiol. 2019 Sep 12;9:321.

    PMID: 31572690

  • Toxoplasma gondii.

    Lourido S.

    Trends Parasitol. 2019 Nov;35(11):944-945.

    PMID: 31345768

  • Optimizing Systems for Cas9 Expression in Toxoplasma gondii.

    Markus BM, Bell GW, Lorenzi HA, Lourido S.

    mSphere. 2019 Jun 26;4(3):e00386-19.

    PMID: 31243081

  • Alveolar proteins stabilize cortical microtubules in Toxoplasma gondii.

    Harding CR, Gow M, Kang JH, Shortt E, Manalis SR, Meissner M, Lourido S.

    Nat Commun. 2019 Jan 23;10(1):401.

    PMID: 30674885

2018 — 4 publications

  • Identification of cryptic subunits from an apicomplexan ATP synthase.

    Huet D, Rajendran E, van Dooren GG, Lourido S.

    Elife. 2018 Sep 11;7:e38097.

    PMID: 30204085

  • Toxoplasma CRISPR/Cas9 constructs are functional for gene disruption in Neospora caninum.

    Arranz-Solís D, Regidor-Cerrillo J, Lourido S, Ortega-Mora LM, Saeij JPJ.

    Int J Parasitol. 2018 Jul;48(8):597-600.

    PMID: 29625127

  • Identification of PNG kinase substrates uncovers interactions with the translational repressor TRAL in the oocyte-to-embryo transition.

    Hara M, Lourido S, Petrova B, Lou HJ, Von Stetina JR, Kashevsky H, Turk BE, Orr-Weaver TL.

    Elife. 2018 Feb 26;7:e33150.

    PMID: 29480805

  • CRISPR-Cas9-based genome-wide screening of Toxoplasma gondii.

    Sidik SM, Huet D, Lourido S.

    Nat Protoc. 2018 Jan;13(1):307-323.

    PMID: 29323662

2016 — 6 publications

  • O-fucosylated glycoproteins form assemblies in close proximity to the nuclear pore complexes of Toxoplasma gondii.

    Bandini G, Haserick JR, Motari E, Ouologuem DT, Lourido S, Roos DS, Costello CE, Robbins PW, Samuelson J.

    Proc Natl Acad Sci U S A. 2016 Oct 11;113(41):11567-11572.

    PMID: 27663739

  • A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes.

    Sidik SM, Huet D, Ganesan SM, Huynh MH, Wang T, Nasamu AS, Thiru P, Saeij JPJ, Carruthers VB, Niles JC, Lourido S.

    Cell. 2016 Sep 8;166(6):1423-1435.e12.

    PMID: 27594426

  • Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and Toxoplasma gondii challenges with a single dose.

    Chahal JS, Khan OF, Cooper CL, McPartlan JS, Tsosie JK, Tilley LD, Sidik SM, Lourido S, Langer R, Bavari S, Ploegh HL, Anderson DG.

    Proc Natl Acad Sci U S A. 2016 Jul 19;113(29):E4133-42.

    PMID: 27382155

  • Serum Albumin Stimulates Protein Kinase G-dependent Microneme Secretion in Toxoplasma gondii.

    Brown KM, Lourido S, Sibley LD.

    J Biol Chem. 2016 Apr 29;291(18):9554-65.

    PMID: 26933037

  • Using a Genetically Encoded Sensor to Identify Inhibitors of Toxoplasma gondii Ca2+ Signaling.

    Sidik SM, Hortua Triana MA, Paul AS, El Bakkouri M, Hackett CG, Tran F, Westwood NJ, Hui R, Zuercher WJ, Duraisingh MT, Moreno SN, Lourido S.

    J Biol Chem. 2016 Apr 29;291(18):9566-80.

    PMID: 26933036

  • Identification of Direct Kinase Substrates Using Analogue-Sensitive Alleles.

    Rothenberg DA, Gordon EA, White FM, Lourido S.

    Methods Mol Biol. 2016;1355:71-84.

    PMID: 26584919

2015 — 4 publications

  • Allosteric activation of apicomplexan calcium-dependent protein kinases.

    Ingram JR, Knockenhauer KE, Markus BM, Mandelbaum J, Ramek A, Shan Y, Shaw DE, Schwartz TU, Ploegh HL, Lourido S.

    Proc Natl Acad Sci U S A. 2015 Sep 8;112(36):E4975-84.

    PMID: 26305940

  • The Toxoplasma Dense Granule Proteins GRA17 and GRA23 Mediate the Movement of Small Molecules between the Host and the Parasitophorous Vacuole.

    Gold DA, Kaplan AD, Lis A, Bett GC, Rosowski EE, Cirelli KM, Bougdour A, Sidik SM, Beck JR, Lourido S, Egea PF, Bradley PJ, Hakimi MA, Rasmusson RL, Saeij JP.

    Cell Host Microbe. 2015 May 13;17(5):642-52.

    PMID: 25974303

  • The calcium signaling toolkit of the Apicomplexan parasites Toxoplasma gondii and Plasmodium spp.

    Lourido S, Moreno SN.

    Cell Calcium. 2015 Mar;57(3):186-93.

    PMID: 25605521

  • One-step enzymatic modification of the cell surface redirects cellular cytotoxicity and parasite tropism.

    Swee LK, Lourido S, Bell GW, Ingram JR, Ploegh HL.

    ACS Chem Biol. 2015 Feb 20;10(2):460-5.

    PMID: 25360987

2014 — 1 publications

  • Efficient genome engineering of Toxoplasma gondii using CRISPR/Cas9.

    Sidik SM, Hackett CG, Tran F, Westwood NJ, Lourido S.

    PLoS One. 2014 Jun 27;9(6):e100450.

    PMID: 24971596

Contact

Lourido Lab
Whitehead Institute for Biomedical Research
Room 661G (Office), Room 657 (Lab)
455 Main Street
Cambridge, MA 02142-1479
Tel.: 617-324-4920 (Office), 617-324-5869 (Lab)

Administrative Lab Manager
Emily Shortt
Tel.: 617-258-5059

Highly motivated individuals should address inquiries about joining our lab to both Sebastian and Emily, along with a CV and the names of references.